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KMID : 0545119990090060820
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 6 p.820 ~ p.825
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Isolation and Characterization of a Novel Exopolysaccharide-Producing Paenibacillus sp.WN9 KCTC 8951P
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Seo, Weon Taek
Kahng, Goon Gjung/Nam, Sang Hae/Choi, Sang Do/Suh, Hyun Hyo/Kim, Seon Won/Park, Yong Ha
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Abstract
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A bacterial strain WN9, which produced a new type of extracellular polysaccharide, was isolated from soil samples. By morphological, physiological, biochemical, and phylogenetic studies, strain WN9 was identified as a Paenibaeillus sp. and it was named as Paenibacillus sp. WN9, which produced a high molecular extracellular polysaccharide from glucose. The molecular weight of the exopolysaccharide (EPS-WN9) was estimated to be about 31.5 mega-Da. The FT-IR spectrum of EPS-WN9 revealed typical characteristics of polysaccharides. EPS-WN9 consisted of D-glucose and D-mannose with a molar ratio of 1:1.4 being identified as a neutral sugar component. The acidic component of EPS-WN9 was tyrosine. Rheological analysis of EPS-WN9 revealed that the pseudoplastic property and its apparent viscosity remained stable at various temperatures and pHs.
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KEYWORD
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